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Original Research
9.
Study on occurrence and antibiogram pattern of
Escherichia coli from raw milk samples in Anand,
Gujarat, India -
H. C. Thaker, M. N. Brahmbhatt, J B Nayak
Vet World. 2012; 5(9): 556-559
doi:
10.5455/vetworld.2012.556-559
Abstract
Aim:
The study was carried out with aim to isolate
Escherichia coli from raw milk samples and
determine antibiogram pattern of E. coli
isolates.
Materials and Methods: During 6
months duration of study a total of 100 raw milk
samples were collected from different places in and
around Anand city such as individual household,
cattle farms, milk collection centres of
Co-operative milk dairies and milk vendors. All raw
milk samples were enriched in peptone water and
inoculated on selective media and various
biochemical tests were performed for confirmation of
isolates. Antibiogram pattern of E. coli to
antimicrobial agents was evaluated by disk diffusion
method. E. coli isolates were sent to
National Salmonella and Escherichia Centre, Central
Research Institute, Kasauli, Himachal Pradesh for
serotyping.
Results: The result of present
study revealed that out of 100 samples, 38 samples
were found contaminated with E. coli.
Antibiogram pattern revealed high resistance against
ampicillin (100%), whereas moderate resistance was
observed for streptomycin (57.89%), oxytetracycline
(47.37%) and amoxy-clav (42.11%). Also lesser
percentage of resistance was observed for co-trimoxazole
(13.16%) and chloramphenicol (5.26%). Serotypes
detected were O24 (7 isolates), followed by O36 (1
isolate), O89 (1 isolate), O91 (1 isolate) and
O153(1 isolate).
Conclusions: Results
suggested a possibility of potential public health
threat of E. coli originating from raw milk
sources.
Keywords:
Antibiogram pattern, E.coli, Enteropathogenic,
Raw milk, Serotypes.
